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Self-assembly of quantum dots/denatured BSA-oligonucleotides bioconjugate and its application on aptameric gold nanoparticles-based biosensor for the determination of rHuEPO-α
- Source :
-
Biosensors & Bioelectronics . May2013, Vol. 43, p446-452. 7p. - Publication Year :
- 2013
-
Abstract
- Abstract: In this paper, denatured bovine serum albumin (dBSA) has been developed as a useful scaffold for appending multiple oligonucleotides (ODNs), and the resulted novel dBSA-ODNs covalent hybrid can be self-assembled to the surface of CdTe quantum dots (QDs) via complexation with unoccupied coordination metal sites on QDs'' surface. The formed QDs/dBSA-ODNs bioconjugate could keep a long-term stability in aqueous solution while maintaining its fluorescence quantum yield and spectral properties. Furthermore, the QDs/dBSA-ODNs also can be readily hybridized with an anti-rHuEPO-α aptamers linked AuNPs (Apt-AuNPs) to form a fluorescent resonance energy transfer-based nano-biosensing system for highly sensitive and selective detection of rHuEPO-α protein lowered to 53.6pM in a rapid, simple manner. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 43
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 85902705
- Full Text :
- https://doi.org/10.1016/j.bios.2012.12.040