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Analysis of the Costructure of the Simian Virus 40 T-Antigen Origin Binding Domain with Site I Reveals a Correlation between GAGGC Spacing and Spiral Assembly.
- Source :
-
Journal of Virology . Mar2013, Vol. 87 Issue 5, p2923-2934. 12p. - Publication Year :
- 2013
-
Abstract
- Polyomavirus origins of replication contain multiple occurrences of G(A/G)GGC, the high-affinity binding element for the viral initiator T-antigen (T-ag). The site I regulatory region of simian virus 40, involved in the repression of transcription and the enhancement of DNA replication initiation, contains two GAGGC sequences arranged head to tail and separated by a 7-bp AT-rich sequence. We have solved a 3.2-Å costructure of the SV40 origin-binding domain (OBD) bound to site I . We have also established that T-ag assembly on site I is limited to the formation of a single hexamer. These observations have enabled an analysis of the role(s) of the OBDs bound to the site I pentanucleotides in hexamer formation. Of interest, they reveal a correlation between the OBDs bound to site I and a pair of OBD subunits in the previously described hexameric spiral structure. Based on these findings, we propose that spiral assembly is promoted by pentanucleotide pairs arranged in a head-to-tail manner. Finally, the possibility that spiral assembly by OBD subunits accounts for the heterogeneous distribution of pentanucleotides found in the origins of replication of polyomaviruses is discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- *POLYOMAVIRUSES
*POLYOMAVIRUS diseases
*ANTIGENS
*DNA replication
*NUCLEOTIDES
Subjects
Details
- Language :
- English
- ISSN :
- 0022538X
- Volume :
- 87
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 86261709
- Full Text :
- https://doi.org/10.1128/JVI.02549-12