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Effects of p,p′-DDE on the mRNA and protein expressions of vimentin, N-cadherin and FSHR in rats testes: An in vivo and in vitro study
- Source :
-
Environmental Toxicology & Pharmacology . May2013, Vol. 35 Issue 3, p486-494. 9p. - Publication Year :
- 2013
-
Abstract
- Abstract: To elucidate the mechanism underlying the testicular effects of 1,1-dichloro-2,2bis(p-chlorophenyl)ethylene (p,p′-DDE), the expressions of vimentin, neural cadherin and follicle-stimulating hormone receptor mRNA and proteins were measured in vivo and in vitro. Sprague-Dawley rats were dosed with p,p′-DDE at 0, 20, 60 and 100mg/kg every other day by intraperitoneal injection for 10 days, and Sertoli cells were treated with p,p′-DDE (0, 10, 30, and 50μM) for 24h. Results indicated that the survival rate of Sertoli cells was decreased with increasing doses of p,p′-DDE. In vitro and in vivo studies, p,p′-DDE could increase the expression of neural cadherin, follicle-stimulating hormone receptor mRNA, while decrease the levels of vimentin, neural cadherin and follicle-stimulating hormone receptor proteins. Moreover, immunohistochemistry analysis revealed that the protein expressions of vimentin, neural cadherin and follicle-stimulating hormone receptor in pubertal rat testes were disrupted by treatment with p,p′-DDE. Taken together, these results suggested that p,p′-DDE exposure could induce testicular toxicity through the changes of the mRNA and protein expressions of vimentin, neural cadherin and follicle-stimulating hormone receptor in vitro and in vivo. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 13826689
- Volume :
- 35
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Environmental Toxicology & Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 86664634
- Full Text :
- https://doi.org/10.1016/j.etap.2013.02.008