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Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages

Authors :
Henrique de Araujo, Tomaz
Okada, Sabrina Sayori
Ghosn, Eliver Eid Bou
Taniwaki, Noemi Nosomi
Rodrigues, Maria Rita
Rogerio de Almeida, Sandro
Mortara, Renato Arruda
Russo, Momtchilo
Campa, Ana
Albuquerque, Renata Chaves
Source :
Cellular Immunology. Jan2013, Vol. 281 Issue 1, p27-30. 4p.
Publication Year :
2013

Abstract

Abstract: Generation of hypochlorous acid (HOCl), an important microbicidal agent, is considered to be the main function of myeloperoxidase (MPO), an enzyme present in phagocytes. High amounts of MPO are present in neutrophil azurophilic granules, which are mobilized into the phagolysosome vacuole during phagocytosis. MPO is also present in monocytes and macrophages, although to a lesser degree than in neutrophils. In the present study, we investigated the distribution of MPO in murine peritoneal cells using flow cytometry, confocal microscopy (CM) and transmission electron microscopy (TEM). MPO was observed in macrophages, and surprisingly, we detected MPO in B lymphocytes, specifically in B1-a. MPO was present in cytoplasmic granules, vesicles, mitochondria and the nucleus of murine peritoneal cells. Together, these findings suggest that, in addition to its known microbicidal activity, MPO has a myriad of other unanticipated cellular functions. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00088749
Volume :
281
Issue :
1
Database :
Academic Search Index
Journal :
Cellular Immunology
Publication Type :
Academic Journal
Accession number :
86809493
Full Text :
https://doi.org/10.1016/j.cellimm.2013.01.002