AN AMPEROMETRIC GLUCOSE BIOSENSOR INCORPORATING A PERMEABLE PRE-OXIDATION LAYER.

An amperometric glucose biosensor based on a coimmobilized electron transfer mediator (osmium-complex) layer, an enzyme (glucose oxidase) layer and a permeable pre-oxidation layer on a glassy carbon electrode was devised. The composition of the pre-oxidation layer was optimized. The permeable cellulose acetate membrane employing lead oxide as an interference-removing agent provided favorable permeability and was insusceptible to interference of ascorbic acid and uric acid. The biosensor exhibited high selectivity and a fast response and allowed a high working potential of +0.55V vs. SCE. The amperometric response to glucose shows a linear relation in the range from 0 to 10 mM with a correlation coefficient of 0.9967. The biosensor was applied to the determination of glucose in human serum. [ABSTRACT FROM AUTHOR]