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Targeted disruption of the α1,3-galactosyltransferase gene in cloned pigs.
- Source :
-
Nature Biotechnology . Mar2002, Vol. 20 Issue 3, p251. 5p. - Publication Year :
- 2002
-
Abstract
- Galactose-α1,3-galactose (α1,3Gal) is the major xenoantigen causing hyperacute rejection in pig-to-human xenotransplantation. Disruption of the gene encoding pig (α1,3-galactosyltransferase (α1,3GT) by homologous recombination is a means to completely remove the α1,3Gal epitopes from xenografts. Here we report the disruption of one allele of the pig α1,3GT gene in both male and female porcine primary fetal fibroblasts. Targeting was confirmed in 17 colonies by Southern blot analysis, and 7 of them were used for nuclear transfer. Using cells from one colony, we produced six cloned female piglets, of which five were of normal weight and apparently healthy. Southern blot analysis confirmed that these five piglets contain one disrupted pig α1,3GT allele. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENES
*GALACTOSYLTRANSFERASES
Subjects
Details
- Language :
- English
- ISSN :
- 10870156
- Volume :
- 20
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Nature Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 8782470
- Full Text :
- https://doi.org/10.1038/nbt0302-251