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Weissellicin L, a novel bacteriocin from sian-sianzih-isolated Weissella hellenica 4-7.
- Source :
-
Journal of Applied Microbiology . Jul2013, Vol. 115 Issue 1, p70-76. 7p. 1 Color Photograph, 2 Charts, 3 Graphs. - Publication Year :
- 2013
-
Abstract
- Aim To identify and characterize novel bacteriocins from Weissella hellenica 4-7. Methods and Results Weissella hellenica 4-7, isolated from the traditional Taiwanese fermented food sian-sianzih (fermented clams), was previously found to produce a bacteriocin active against Listeria monocytogenes and some other Gram-positive bacteria. Bacteriocin activity decreased slightly after autoclaving (121°C for 15 min), but was inactivated by protease K and trypsin. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 3205·6 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2- KGFLSWASKATSWLVGP, by Edman degradation. The obtained partial sequence showed high homology with leucocin B- TA33a; however, at least two different residues were observed. No identical peptide or protein was found, and this peptide was therefore considered to be a novel bacteriocin produced by W. hellenica 4-7 and termed weissellicin L. Conclusions The findings obtained in the current study suggest a novel bacteriocin produced by W. hellenica 4-7. Significance and Impact of the Study Bacteriocins from Weissella remain rare, and this study is the second report of a bacteriocin produced by W. hellenica. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13645072
- Volume :
- 115
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Applied Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 88155657
- Full Text :
- https://doi.org/10.1111/jam.12218