Cholinergic enhancement by pyridostigmine increases the insulin response to glucose load in obese patients but not in normal subjects.

OBJECTIVES: To further investigate the role, if any, of acetylcholine and the parasympathetic nervous system in modulating β-cell secretion in man. DESIGN: Oral glucose load (OGTT, 100 g p.o. at 0 min ) alone and preceded by pyridostigmine (PD, 120 mg p.o., 60 min before OGTT), a cholinesterase inhibitor, were administered on two different occasions, in random order, two or three days apart. SUBJECTS: Ten women with central obesity (OB, body mass index(BMI): 34.3±2.1 kg/m2, waist to hip ratio (WHR): 0.83±0.01, aged 39.0±5.3 y) and six normal women (NS, BMI: 22.7±1.9 kg/m2, WHR: 0.74±0.01, aged 37.1±4.8 y) were studied. MEASUREMENTS: Serum insulin, plasma glucose and plasma noradrenaline (NA) were measured at -60, -15 and 0 min, and then every 15 min up to +120 min. Insulin concentrations were measured in duplicate by immunoradiometric assay, glucose by glucose oxidase colorimetric method and NA was assayed after extraction with alumina using high performance liquid chromatography with electrochemical detection. Pulse rate (PR), systolic (SBP) and diastolic blood pressure (DBP) were also measured every 15 min during the tests by an automated cuff device. RESULTS: OGTT raised glucose concentrations in OB and NS (incremental area: 420±44 vs 288±70 mmol/l. 2 h, respectively) without significant differences between groups(F=0.6, P=ns). On the other hand, OB showed an insulin response to OGTT higher than NS (10 120±1074 vs 6692±1962 μU ml-1 2 h, respectively P<0.01). After OGTT alone NA concentrations increased to the same extent in NS (peak vs basal: 1.40±0.16 vs 1.07±0.10 nmol/l, P<0.05) and in OB (peak vs basal: 1.50±0.14 vs 1.04±0.18 nmol/l P<0.05). Both in NS and in OB, PD administration failed to modify basal glucose and insulin (P=ns for both) as well as basal NA concentrations. In NS, the combined administration of PD and OGTT did not modify... [ABSTRACT FROM AUTHOR]