Determination of stage interconversion in vitro and in vivo by construction of transgenic Toxoplasma gondii that stably express stage-specific fluorescent proteins.

Highlights: [•] A transgenic cyst-forming strain (PLK) of Toxoplasma gondii was constructed. [•] The parasites stably expressed green fluorescence protein in the tachyzoite stage. [•] The parasites stably expressed red fluorescence protein in the bradyzoite stage. [•] The parasites can be used for in vitro and in vivo studies on stage conversion. [ABSTRACT FROM AUTHOR]