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Lentiviral Vector-Mediated siRNA Knockdown of c-MYC: Cell Growth Inhibition and Cell Cycle Arrest at G2/M Phase in Jijoye Cells.
- Source :
-
Biochemical Genetics . Aug2013, Vol. 51 Issue 7/8, p603-617. 15p. - Publication Year :
- 2013
-
Abstract
- Inhibition of c-MYC has been considered as a potential therapy for lymphoma treatment. We explored a lentiviral vector-mediated small interfering RNA (siRNA) expression vector to stably reduce c-MYC expression in B cell line Jijoye cells and investigated the effects of c-MYC downregulation on cell growth, cell cycle, and apoptosis in vitro. The expression of c-MYC mRNA and protein levels were inhibited significantly by c-MYC siRNA. The c-MYC downregulation resulted in the inhibition of cell proliferation and cell cycle arrest at G2/M phase, which was associated with decreased expression of cyclin B and cyclin-dependent kinase 1 (CDK1) and increased expression of CDK inhibitor p21 proteins. In addition, downregulation of c-MYC induced cell apoptosis characterized by DNA fragmentation and caspase-3 activation. Taken together, these results suggest that lentiviral vector-mediated siRNA for c-MYC may be a promising approach for targeting c-MYC in the treatment of Burkitt lymphoma. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00062928
- Volume :
- 51
- Issue :
- 7/8
- Database :
- Academic Search Index
- Journal :
- Biochemical Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 89046644
- Full Text :
- https://doi.org/10.1007/s10528-013-9590-0