Isolation, identification and characterization of a Peste des Petits Ruminants virus from an outbreak in Nanakpur, India.

Highlights: [•] Peste des Petits Ruminants virus was isolated from a field outbreak in India. [•] Identity of the virus was confirmed by amplification and sequence analysis of N gene. [•] Virus reacted poorly with the diagnostic reagents used for PPR diagnosis in India. [•] A plaque assay for PPR virus was first time established. [•] In one step growth curve, duration of PPRV life cycle was determined (6–8h). [Copyright &y& Elsevier]