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Extracellular regulated kinase (ERK)-dependent regulation of sialomucin complex/rat Muc4 in mammary epithelial cells.
- Source :
-
Oncogene . 9/7/2000, Vol. 19 Issue 38, p4354. 8p. - Publication Year :
- 2000
-
Abstract
- Sialomucin complex (SMC, rat Muc4) is a membrane mucin implicated in the protection of epithelia and the metastasis of some tumors. It is a heterodimeric complex, containing a mucin subunit with anti-adhesive activity and a transmembrane subunit with epidermal growth factor-like domains, one of which acts as an intramembrane ligand for ErbB2. Serum, insulin and insulin-like growth factor, but not epidermal growth factor, induce the expression of sialomucin complex in mammary epithelial cells. Induction correlates with sustained, but not transient, activation of extracellular-regulated protein kinase (ERK). MEK inhibitor U0126 blocked the induction, while activated MEK-1 transfected into a rat mammary adenocarcinoma cell line induced a sustained activation of ERK and up-regulated SMC/Muc4 expression. Northern and Western blotting indicated that up-regulation occurred concomitantly at the transcript and protein levels, both of which could be blocked by U0126. These results suggest that expression of SMC/Muc4 in mammary epithelial cells is regulated by selected growth factors through an ERK-dependent pathway at the transcript level. Oncogene (2000) 19, 4354–4361 [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEIN kinases
*EPITHELIAL cells
*MAMMARY glands
*GLYCOPROTEINS
Subjects
Details
- Language :
- English
- ISSN :
- 09509232
- Volume :
- 19
- Issue :
- 38
- Database :
- Academic Search Index
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 8911307
- Full Text :
- https://doi.org/10.1038/sj.onc.1203781