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Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus.
- Source :
-
Oncogene . 1/25/2001, Vol. 20 Issue 4, p523. 8p. - Publication Year :
- 2001
-
Abstract
- Viral interferon regulatory factor (vIRF) encoded by Kaposi's sarcoma-associated herpesvirus (KSHV) inhibits the expression of interferon-responsive genes, causes cellular transformation and transactivates KSHV genes. In the present study, we characterized the mRNA expression pattern of the vIRF gene and its promoter. A vIRF transcript of 1.7 kb in size was detected in low level in uninduced KSHV-infected cells and its expression was inducible by 12-O-tetradecanoylphorbol-13-acetate (TPA), sensitive to cycloheximide and resistant to phosphonoacetic acid. The transcription start site was mapped to 79 nt upstream of the ATG initiation site by 5′-RACE. Mutagenesis analysis identified a region between -56 and the transcription start site (+1) as the minimal promoter region that contains a functional TATA box at -27. A region between -337 and -125 contains a repressor domain negated by sequence from -991 to -499 in BCBL-1 cells, a region which was also identified to be responsive to TPA induction. These results demonstrate vIRF as a KSHV early gene, identify its promoter and define the promoter regions that contain regulatory elements controlling vIRF transcription. Oncogene (2001) 20, 523–530. [ABSTRACT FROM AUTHOR]
- Subjects :
- *KAPOSI'S sarcoma
*ONCOGENES
*MUTAGENESIS
Subjects
Details
- Language :
- English
- ISSN :
- 09509232
- Volume :
- 20
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Oncogene
- Publication Type :
- Academic Journal
- Accession number :
- 8919129
- Full Text :
- https://doi.org/10.1038/sj.onc.1204115