Enzyme activity measurement via spectral evolution profiling and PARAFAC.

Highlights: [•] Enzyme activity determination using FTIR and PARAFAC. [•] Directly and universally applicable without the need of any external standards. [•] The method provides alternative solutions to the cumbersome reducing sugar analysis. [•] Enzymatic hydrolysis of plant polymers and sugar release can be determined. [Copyright &y& Elsevier]