Pharmacokinetic comparisons of puerarin, daidzin and the glucuronide metabolite of puerarin after administration of total flavonoid from Gegen alone and total flavonoid from Gegen combined with total saponin from Sanqi in rats under different physiological states.

Highlights: [•] Simultaneous determination of puerarin glucuronide, puerarin and daidzin in rat plasma. [•] An UPLC–MS/MS method was developed and applied for pharmacokinetic study. [•] Comparative pharmacokinetics was performed in rats under different physiological states. [•] Blood stasis syndrome had effect on pharmacokinetics of puerarin glucuronide, puerarin and daidzin. [•] Pharmacokinetics of the three constituents changed when Gegen was used in combination with Sanqi. [Copyright &y& Elsevier]