Purification of phosphinothricin acetyltransferase using Reactive brown 10 affinity in a single chromatography step.

Highlights: [•] One step purification of phosphinothricin acetyltransferase (PAT) is developed. [•] The purification employs an affinity chromatography using Reactive brown 10. [•] The method is simple, time-saving, inexpensive and reproducible. [•] The method enables to obtain pure PAT from various sources. [•] Other applications of the method are also discussed. [Copyright &y& Elsevier]