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Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay.
- Source :
-
Chemistry Central Journal . 2013, Vol. 7 Issue 1, p1-8. 8p. - Publication Year :
- 2013
-
Abstract
- Background: A label-free immunosensor from as-grown double wall carbon nanotubes (DW) bundles was developed for detecting Salmonella typhimurium. The immunosensor was fabricated by using the as-grown DW bundles as an electrode material with an anti-Salmonella impregnated on the surface. The immunosensor was electrochemically characterized by cyclic voltammetry. The working potential (100, 200, 300 and 400 mV vs. Ag/AgCl) and the anti-Salmonella concentration (10, 25, 50, 75, and 100 μg/mL) at the electrode were subsequently optimized. Then, chronoamperometry was used with the optimum potential of 100 mV vs. Ag/AgCl) and the optimum impregnated anti-Salmonella of 10 μg/mL to detect S. typhimurium cells (0-109 CFU/mL). Results: The DW immunosensor exhibited a detection range of 102 to 107 CFU/mL for the bacteria with a limit of detection of 8.9 CFU/mL according to the IUPAC recommendation. The electrode also showed specificity to S. typhimurium but no current response to Escherichia coli. Conclusions: These findings suggest that the use of a label-free DW immunosensor is promising for detecting S. typhimurium. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1752153X
- Volume :
- 7
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Chemistry Central Journal
- Publication Type :
- Academic Journal
- Accession number :
- 89687639
- Full Text :
- https://doi.org/10.1186/1752-153X-7-102