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Enterocin TW21, a novel bacteriocin from dochi-isolated Enterococcus faecium D081821.
- Source :
-
Journal of Applied Microbiology . Sep2013, Vol. 115 Issue 3, p673-678. 6p. 1 Color Photograph, 2 Diagrams, 2 Graphs. - Publication Year :
- 2013
-
Abstract
- Aims Purification and characterization of a novel bacteriocin produced by strain Enterococcus faecium D081821. Methods and Results Enterococcus faecium D081821, isolated from the traditional Taiwanese fermented food dochi (fermented black beans), was previously found to produce a bacteriocin against Listeria monocytogenes and some Gram-positive bacteria. This bacteriocin, termed enterocin TW21, was purified from culture supernatant by ammonium sulfate precipitation, Sep- Pak C18 cartridge, ion-exchange and gel filtration chromatography. Mass spectrometry analysis showed the mass of the peptide to be approximately 5300·6 Da. The N-terminal amino acid sequencing yielded a partial sequence NH2- ATYYGNGVYx NTQK by Edman degradation, and it contains the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The open reading frame ( ORF) encoding the bacteriocin was identified from the draft genome sequence of Enterococcus faecium D081821, and sequence analysis of this peptide indicated that enterocin TW21 is a novel bacteriocin. Conclusions Enterococcus faecium D081821 produced a bacteriocin named enterocin TW21, the molecular weight and amino acid sequence both revealed it to be a novel bacteriocin. Significant and Impact of Study A new member of class IIa bacteriocin was identified. This bacteriocin shows great inhibitory ability against L. monocytogenes and could be applied as a natural food preservative. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13645072
- Volume :
- 115
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Applied Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 89703731
- Full Text :
- https://doi.org/10.1111/jam.12265