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Sox10-MCS5 enhancer dynamically tracks human oligodendrocyte progenitor fate.
- Source :
-
Experimental Neurology . Sep2013, Vol. 247, p694-702. 9p. - Publication Year :
- 2013
-
Abstract
- Abstract: In this study, we sought to establish a novel method to prospectively and dynamically identify live human oligodendrocyte precursor cells (OPCs) and oligodendrocyte lineage cells from brain dissociates and pluripotent stem cell culture. We selected a highly conserved enhancer element of the Sox10 gene, known as MCS5, which directs reporter expression to oligodendrocyte lineage cells in mouse and zebrafish. We demonstrate that lentiviral Sox10-MCS5 induced expression of GFP at high levels in a subpopulation of human CD140a/PDGFαR-sorted OPCs as well as their immature oligodendrocyte progeny. Furthermore, we show that almost all Sox10-MCS5:GFPhigh cells expressed OPC antigen CD140a and human OPCs expressing SOX10, OLIG2, and PDGFRA mRNAs could be prospectively identified using GFP based fluorescence activated cells sorting alone. Additionally, we established a human induced pluripotent cell (iPSC) line transduced with the Sox10-MCS5:GFP reporter using a Rex-Neo cassette. Similar to human primary cells, GFP expression was restricted to embryoid bodies containing both oligodendrocyte progenitor and oligodendrocyte cells and co-localized with NG2 and O4-positive cells respectively. As such, we have developed a novel reporter system that can track oligodendrocyte commitment in human cells, establishing a valuable tool to improve our understanding and efficiency of human oligodendrocyte derivation. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00144886
- Volume :
- 247
- Database :
- Academic Search Index
- Journal :
- Experimental Neurology
- Publication Type :
- Academic Journal
- Accession number :
- 89704828
- Full Text :
- https://doi.org/10.1016/j.expneurol.2013.03.010