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Prokaryotic Expression of F Protein from PPRV and Characterization of Its Polyclonal Antibody.
- Source :
-
Monoclonal Antibodies in Immunodiagnosis & Immunotherapy . Feb2013, Vol. 32 Issue 1, p26-31. 6p. - Publication Year :
- 2013
-
Abstract
- The goal of this study was to evaluate the specificity of a polyclonal antibody against the F protein from Peste des petits ruminants virus (PPRV). A pET30a/F prokaryotic expression vector was successfully constructed and its recombinant protein was expressed. The result of Western blot analysis showed that the fusion protein pET30a/F possessed good immunoreactivity and the purified recombinant protein was then used as the antigen to raise anti-pET30a/F polyclonal antibody in rabbits. The polyclonal antibody titer against the recombinant F protein was confirmed by indirect ELISA, and the protein's specificity against pET30/F polyclonal antibody was confirmed by both Western blot and indirect immunofluorescence assay in transfected cells. In short, we obtained the high-level expression of recombinant F protein as well as high titers of rabbit polyclonal antibody specificity against F protein in pCAGGS/F transfected cells. This special polyclonal antibody offers a valuable and useful tool for further study of the pathogenesis of PPRV early infection and the structural and functional characterization of PPRV F protein. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21679436
- Volume :
- 32
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Monoclonal Antibodies in Immunodiagnosis & Immunotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 89859954
- Full Text :
- https://doi.org/10.1089/mab.2012.0063