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The Evolutionary Divergence of Shiga Toxin-Producing Escherichia coli Is Reflected in Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Spacer Composition.
- Source :
-
Applied & Environmental Microbiology . Sep2013, Vol. 79 Issue 18, p5710-5720. 11p. - Publication Year :
- 2013
-
Abstract
- The Shiga toxin-producing Escherichia coli (STEC) strains, including those of 0157:H7 and the "big six" serogroups (i.e., sero-groups 026,045,0103, Olll, 0121, and 0145), are a group of pathogens designated food adulterants in the United States. The relatively conserved nature of clustered regularly interspaced short palindromic repeats (CRISPRs) in phylogenetically related E. coli strains makes them potential subtyping markers for STEC detection, and a quantitative PCR (qPCR)-based assay was previously developed for 026:H11,045:H2,0103:H2, OHl:H8,0121:H19,0145:H28, and 0157:H7 isolates. To better evaluate the sensitivity and specificity of this qPCR method, the CRISPR loci of 252 0157 and big-six STEC isolates were sequenced and analyzed along with 563 CRISPR1 and 624 CRISPR2 sequences available in GenBank. General conservation of spacer content and order was observed within each 0157 and big-six serogroup, validating the qPCR method. Meanwhile, it was found that spacer deletion, the presence of an insertion sequence, and distinct alleles within a serogroup are sources of false-negative reactions. Conservation of CRISPR arrays among isolates expressing the same flagellar antigen, specifically, H7, H2, and Hll, suggested that these isolates share an ancestor and provided an explanation for the false positives previously observed in the qPCR results. An analysis of spacer distribution across E. coli strains provided limited evidence for temporal spacer acquisition. Conversely, comparison of CRISPR sequences between strains along the stepwise evolution of 0157:H7 from its 055:H7 ancestor revealed that, over this ~ 7,000-year span, spacer deletion was the primary force generating CRISPR diversity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00992240
- Volume :
- 79
- Issue :
- 18
- Database :
- Academic Search Index
- Journal :
- Applied & Environmental Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 90060160
- Full Text :
- https://doi.org/10.1128/AEM.00950-13