A novel ion pairing LC/MS metabolomics protocol for study of a variety of biologically relevant polar metabolites.

Highlights: [•] We developed a novel IP-LC–MS protocol for the identification and quantification of polar metabolites. [•] The IP method resolves biologically interesting molecules with good linearity and repeatability. [•] We demonstrate the versatility of method by determining the metabolomic signature of 3 biological systems. [ABSTRACT FROM AUTHOR]