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Charge transfer through DNA/DNA duplexes and DNA/RNA hybrids: Complex theoretical and experimental studies.
- Source :
-
Biophysical Chemistry . Oct2013, Vol. 180-181, p127-134. 8p. - Publication Year :
- 2013
-
Abstract
- Abstract: Oligonucleotides conduct electric charge via various mechanisms and their characterization and understanding is a very important and complicated task. In this work, experimental (temperature dependent steady state fluorescence spectroscopy, time-resolved fluorescence spectroscopy) and theoretical (Density Functional Theory) approaches were combined to study charge transfer processes in short DNA/DNA and RNA/DNA duplexes with virtually equivalent sequences. The experimental results were consistent with the theoretical model — the delocalized nature of HOMO orbitals and holes, base stacking, electronic coupling and conformational flexibility formed the conditions for more effective short distance charge transfer processes in RNA/DNA hybrids. RNA/DNA and DNA/DNA charge transfer properties were strongly connected with temperature affected structural changes of molecular systems — charge transfer could be used as a probe of even tiny changes of molecular structures and settings. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 03014622
- Volume :
- 180-181
- Database :
- Academic Search Index
- Journal :
- Biophysical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 90213804
- Full Text :
- https://doi.org/10.1016/j.bpc.2013.07.009