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MLST revisited: the gene-by-gene approach to bacterial genomics.
- Source :
-
Nature Reviews Microbiology . Oct2013, Vol. 11 Issue 10, p728-736. 9p. - Publication Year :
- 2013
-
Abstract
- Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17401526
- Volume :
- 11
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Nature Reviews Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 90246247
- Full Text :
- https://doi.org/10.1038/nrmicro3093