Crystallization and Preliminary X-Ray Crystallographic Analysis of PAS Factor from Vibrio vulnificus.

Plasmid Achromobacter secretion (PAS) factor is a putative secretion factor that induces the secretion of periplasmic proteins. PAS factor from Vibrio vulnificus was crystallized at 294 K by the hanging drop vapor- diffusion method. It was isolated as a monomer during the purification procedures. The native crystal belongs to the F222 space group with unit cell parameters a = 56.1, b = 74.4, c = 80.0 Å, α = β = γ = 90°. The crystal was soaked in cryoprotectant containing 1 M NaBr for 1 h for MAD phasing. The diffraction limit of the Br-MAD data set was 1.9 Å using synchrotron Xray irradiation at beam line BL-18B at the Photon Factory, Japan. [ABSTRACT FROM AUTHOR]