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Knockdown of OY-TES-1 by RNAi causes cell cycle arrest and migration decrease in bone marrow-derived mesenchymal stem cells.
- Source :
-
Cell Biology International . Oct2012, Vol. 36 Issue 10, p917-922. 6p. - Publication Year :
- 2012
-
Abstract
- OY-TES-1 is a member of the CTA (cancer-testis antigen) group expressed in a variety of cancer and restrictedly expressed in adult normal tissues, except for testis. To determine whether MSCs (mesenchymal stem cells) express OY-TES-1 and its possible roles on MSCs, OY-TES-1 expression in MSCs isolated from human bone marrow was tested with RT (reverse transcription)-PCR, immunocytochemistry and Western blot. Using RNAi (RNA interference) technology, OY-TES-1 expression was knocked down followed by analysing cell viability, cell cycle, apoptosis and migration ability. MSCs expressed OY-TES-1 at both mRNA and protein levels. The down-regulation of OY-TES-1 expression in these MSCs caused cell growth inhibition, cell cycle arrest, apoptosis induction and migration ability attenuation. Through these primary results it was suggested that OY-TES-1 may influence the biological behaviour of MSCs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10656995
- Volume :
- 36
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Cell Biology International
- Publication Type :
- Academic Journal
- Accession number :
- 90351910
- Full Text :
- https://doi.org/10.1042/CBI20120037