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Purification and Biochemical Characterization of pH Tolerant and Acid Stable α-amylase from Aspergillus oryzae JGI 21 Isolated from Soil.
- Source :
-
Croatian Journal for Food Technology, Biotechnology & Nutrition / Hrvatski Casopis za Prehrambenu Tehnologiju, Biotehnologiju i Nutricionizam . Jun2013, Vol. 8 Issue 1/2, p14-20. 7p. - Publication Year :
- 2013
-
Abstract
- This paper describes the purification and characterization of a novel acid stable and pH tolerant α-amylase from a Aspergillusoryzae JGI 21 isolated from Mangalore. The enzyme displayed a molecular weight of 22 kDa and it was stable over a broad range of acidic and alkaline pH with maximum activity and stability at 6.5. The optimum temperature of enzyme stability was found to be around 24+/-2°C. The purification of α-amylase by ammonium sulphate precipitation and ion-exchange chromatography resulted in 23.56 fold increase in its activity (100.38 U/mg protein). Considering its promising properties, this enzyme can find potential applications in the food industry as well as in laundry detergents. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 18473423
- Volume :
- 8
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Croatian Journal for Food Technology, Biotechnology & Nutrition / Hrvatski Casopis za Prehrambenu Tehnologiju, Biotehnologiju i Nutricionizam
- Publication Type :
- Academic Journal
- Accession number :
- 90488240