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One single-tube PCR assay to detect both CD17 (A>T) and IVS-II nt-654 (C>T) homozygous mutations of β-thalassemia.
- Source :
-
Turkish Journal of Medical Sciences . Oct2013, Vol. 43 Issue 5, p764-767. 4p. - Publication Year :
- 2013
-
Abstract
- Aim: Several methods can be used to detect gene mutations associated with β-thalassemia, but it is difficult to achieve reliable and reproducible results. In this study, we introduce a new method, a single-tube multiplex polymerase chain reaction (PCR) assay, to detect both CD17 (A>T) and IVS-II nt-654 (C>T) homozygous mutations. Materials and methods: This new method designs specific primers to diagnose homozygous mutations and normal controls. Results: After PCR amplification, homozygous mutations produce different fragments from normal controls. Conclusion: This study represents an important step towards the development of a novel protocol to diagnose β-thalassemia and other diseases that target numerous mutations. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13000144
- Volume :
- 43
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Turkish Journal of Medical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 90518625
- Full Text :
- https://doi.org/10.3906/sag-1209-60