Development, optimization and application of an analytical methodology by ultra performance liquid chromatography–tandem mass spectrometry for determination of amanitins in urine and liver samples.

Highlights: [•] Unequivocal confirmation and determination of α- and β-amanitins in urine and liver samples. [•] Structural characterization of the fragmentation pattern for both amatoxins. [•] LOD and LOQ in urine are much lower compared to previous reports. [•] Diagnostic tool for mushroom intoxication. [Copyright &y& Elsevier]