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A morphologic study of filamentous phage infection of Escherichia coli using biotinylated phages
- Source :
-
FEBS Letters . Feb2003, Vol. 536 Issue 1-3, p167. 6p. - Publication Year :
- 2003
-
Abstract
- Using biotinylated phage (BIO-phages), we observed the infection of filamentous phages into Escherichia coli JM109 morphologically. BIO-phages and BIO-phage-derived proteins, mainly pVIII, were detected in E. coli by using the avidin–biotin–peroxidase complex method with electron microscopy. Infected cells revealed positive staining on the outer and inner membranes and in the periplasmic space. Some cells showed specific or predominant staining of the outer membrane, whereas others showed predominant staining of the inner membrane or equivalent staining of the outer and inner membranes. The periplasmic spaces in some infected cells were expanded and filled with reaction products. Some cells showed wavy lines of positive staining in the periplasmic space. BIO-phages were detected as thick filaments or clusters covered with reaction products. The ends of the infecting phages were located on the surface of cells, in the periplasmic space, or on the inner membrane. These findings suggest that phage major coat proteins are integrated into the outer membrane and that phages cause periplasmic expansion during infection. [Copyright &y& Elsevier]
- Subjects :
- *BACTERIOPHAGES
*ESCHERICHIA coli
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 536
- Issue :
- 1-3
- Database :
- Academic Search Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 9053428
- Full Text :
- https://doi.org/10.1016/S0014-5793(03)00050-4