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Cytotoxic Effect and Induction of Apoptosis in Human Cervical Cancer Cells by Antrodia camphorata.

Authors :
Yang, Pei-Yu
Hu, Dan-Ning
Liu, Fu-Shing
Source :
American Journal of Chinese Medicine. 2013, Vol. 41 Issue 5, p1169-1180. 12p. 1 Chart, 6 Graphs.
Publication Year :
2013

Abstract

Antrodia camphorata is a Chinese herb indigenous to Taiwan. Previous reports demonstrated that it could induce apoptosis in some cancer cells. The purpose of this study was to investigate the apoptotic effect of the crude extract of A. camphorata in cervical cancer cells. Two human cervical cancer cell lines, and C-33A, were treated with extract of A. camphorata (10-1000 μg/mL). We found that A. camphorata extract was cytotoxic to both cervical cancer cells in a dose- and time-dependent manner as examined by MTT assay. Treatment with A. camphorata extract at 400 μg/mL induced a 2.3- and 4.4-fold increase in oligonucleosome formation from the cleaved chromosomal DNA in and C-33A cells, respectively. A. camphorata extract also activated caspase-3, -8, and -9 activities and increased the cytosolic level of cytochrome c in both cell lines as the dosage increased. Furthermore, A. camphorata extract increased expressions of Bak, Bad and Bim, while decreasing expressions of Bcl-2 and Bcl-xL of the Bcl-2 family proteins in and C-33A cells. The expression of IAP proteins, XIAP and survivin, was also decreased in both cervical cancer cells after treatment with A. camphorata. Our in vitro study suggests that A. camphorata is cytotoxic to cervical cancer cells through both extrinsic and intrinsic apoptotic mechanisms. It could be used as a novel phytotherapeutic agent or auxiliary therapy in the treatment of cervical cancer. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0192415X
Volume :
41
Issue :
5
Database :
Academic Search Index
Journal :
American Journal of Chinese Medicine
Publication Type :
Academic Journal
Accession number :
90671893
Full Text :
https://doi.org/10.1142/S0192415X13500791