HighlyEfficient Biodecolorization/Degradation ofCongo Red and Alizarin Yellow R by Chloroperoxidase from Caldariomyces fumago: Catalytic Mechanism and DegradationPathway.

Chloroperoxidase(CPO) from Caldariomyces fumagocouldmediate a very efficient oxidative decolorization/degradationof aqueous azo dye. The decolorization efficiencies for Congo Redand Alizarin Yellow R reached 88.25% in 3 min and 92.38% in 5 min,respectively, under mild conditions at enzyme concentrations of 0.025and 0.03 μmol L–1, respectively. Comparedwith the typical mechanism involved in oxidative decolorization ofazo dyes catalyzed by other peroxidases, a different enzymatic catalyticcycle was found in this work. The oxidative decolorization was carriedout in bulk solution, which would get rid of the restriction of substraterequired by the channel access to the substrate pocket in the enzymaticactive site. Nine degradation products of Congo Red were detectedby HPLC–MS analysis, and two types of degradation pathwayswere proposed accordingly. The strong toleration of the typical saltspecies in industrial effluents by this system ensured that it haspotential applications in treatment of industrial wastewater. [ABSTRACT FROM AUTHOR]