A high-throughput 2D-analytical technique to obtain single protein parameters from complex cell lysates for in silico process development of ion exchange chromatography.

Highlights: [•] Characterization of complex protein mixtures. [•] Multidimensional analysis applying high-throughput techniques. [•] Estimation of single protein chromatograms from multidimensional analysis. [•] Determination of linear steric-mass action parameters for the single proteins. [•] Retention volume prediction and chromatogram simulation using obtained parameters. [Copyright &y& Elsevier]