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Protective effects of oral immunization with live Lactococcus lactis expressing Eimeria tenella 3-1E protein.
- Source :
-
Parasitology Research . Dec2013, Vol. 112 Issue 12, p4161-4167. 7p. - Publication Year :
- 2013
-
Abstract
- The codon-optimized Eimeria tenella 3-1E gene was introduced into the lactic acid bacterial vector pTX8048 to construct plasmid pTX8048-3-1E. The plasmid pTX8048-3-1E was transformed into Lactococcus lactis NZ9000 by electroporation to create the strain of L. lactis pTX8048-3-1E. The expression of objective protein was verified by Western blot. The live bacteria L. lactis pTX8048-3-1E were administered orally, and an animal challenge experiment was carried out to evaluate the protective efficacy. The results indicated the strain of L. lactis pTX8048-3-1E was constructed successfully. Oral immunization to specific pathogen-free (SPF) chickens with L. lactis pTX8048-3-1E provided partial protection against homologous challenge including significant increased oocyst decrease ratio, reduced average lesion score in cecum, and improved body weight gain compared to control bacteria L. lactis pTX8048. These results demonstrate the use of Lactococcus as live vector for delivery of Eimeria antigen is feasible and promising method to control coccidiosis in poultry. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09320113
- Volume :
- 112
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Parasitology Research
- Publication Type :
- Academic Journal
- Accession number :
- 91988743
- Full Text :
- https://doi.org/10.1007/s00436-013-3607-9