Identification and characterization of ΦH111-1.

Characterization of prophages in sequenced bacterial genomes is important for virulence assessment, evolutionary analysis, and phage application development. The objective of this study was to identify complete, inducible prophages in the cystic fibrosis (cF) clinical isolate Burkholderia cenocepacia h111. Using the prophage-finding program Phage search Tool (PhasT), we identified three putative intact prophages in the h111 sequence. Virions were readily isolated from h111 culture supernatants following extended incubation. Using shotgun cloning and sequencing, one of these virions (designated Φh111-1 [vB_BceM_Φh111-1]) was identified as the infective particle of a PhasT-detected intact prophage. Φh111-1 has an extremely broad host range with respect to B. cenocepacia strains and is predicted to use lipopolysaccharide (LPs) as a receptor. Bioinformatics analysis indicates that the prophage is 42,972 base pairs in length, encodes 54 proteins, and shows relatedness to the virion morphogenesis modules of AcaML1 and "Vhmllikevirus" myoviruses. as Φh111-1 is active against a broad panel of clinical strains and encodes no putative virulence factors, it may be therapeutically effective for Burkholderia infections. [ABSTRACT FROM AUTHOR]