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Evaluation of rapid and simple techniques for the enrichment of viruses prior to metagenomic virus discovery.

Authors :
Hall, Richard J.
Wang, Jing
Todd, Angela K.
Bissielo, Ange B.
Yen, Seiha
Strydom, Hugo
Moore, Nicole E.
Ren, Xiaoyun
Huang, Q. Sue
Carter, Philip E.
Peacey, Matthew
Source :
Journal of Virological Methods. Jan2014, Vol. 195, p194-204. 11p.
Publication Year :
2014

Abstract

Highlights: [•] The effect of simple virus enrichment methods were tested on a metagenomics dataset. [•] Centrifugation, filtration or nuclease-treatment was evaluated. [•] A multi-step enrichment method increased the proportion of virus sequences. [•] This evaluation guides researchers in their choice of enrichment methodology. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01660934
Volume :
195
Database :
Academic Search Index
Journal :
Journal of Virological Methods
Publication Type :
Academic Journal
Accession number :
92651166
Full Text :
https://doi.org/10.1016/j.jviromet.2013.08.035