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A novel fluorescence assay for the detection of hemoglobin based on the G-quadruplex/hemin complex.
- Source :
-
Talanta . Jan2014, Vol. 118, p118-122. 5p. - Publication Year :
- 2014
-
Abstract
- Abstract: A novel sensing strategy for specific recognition of hemoglobin has been designed. In the presence of trypsin, hemoglobin was cleaved to release heme which could effectively bind to a FITC-labeled guanine (G)-rich oligonucleotide sequence to form the G-quadruplex/hemin complex. This binding process would lead to fluorescence quenching of FITC via photoinduced electron transfer. Therefore, a fluorescence assay for the determination of hemoglobin could be established. Under the optimized conditions, there was a linear relationship between the fluorescence intensity of probe DNA and the hemoglobin concentration in the range of 5–200nmol/L, with the limit of detection (LOD) as low as 2nmol/L. [Copyright &y& Elsevier]
- Subjects :
- *FLUORESCENCE
*HEMOGLOBINS
*HEMIN
*CHEMICAL detectors
*TRYPSIN
*BIOLOGICAL assay
Subjects
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 118
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 92651519
- Full Text :
- https://doi.org/10.1016/j.talanta.2013.10.004