Simultaneous determination of probe drugs, metabolites, inhibitors and inducer in human plasma by liquid chromatography/tandem mass spectrometry and its application to pharmacokinetic study.

Highlights: [•] We developed a rapid and sensitive liquid chromatography–mass spectrometry method. [•] We simultaneous quantified midazolam, raltegravir and their major metabolites. [•] We also quantified rifampicin (inducer), ritonavir and ketoconazole (inhibitors). [•] We applied this method in a clinical study in Singapore to probe CYP3A and UGT1A1. [ABSTRACT FROM AUTHOR]