Repair kinetics of acrolein- and (E)-4-hydroxy-2-nonenal-derived DNA adducts in human colon cell extracts.

Highlights: [•] The repair kinetics of two common cyclic DNA adducts derived lipid peroxidation, Acr-dG and HNE-dG, in human colon cells was studied. [•] This study is the first to measure DNA repair rates by both DNA repair synthesis and LC–MS/MS methods. [•] Acr-dG, like HNE-dG, is repaired by NER pathway, but it is repaired at a much slower rate compared to HNE-dG. [•] HNE-dG can inhibit the repair of Acr-dG if both are present in the same DNA. [ • ] These results provide an explanation for the higher levels of Acr-dG than HNE-dG observed in vivo. [ABSTRACT FROM AUTHOR]