Transfection of pseudouridine-modified mRNA encoding CPD-photolyase leads to repair of DNA damage in human keratinocytes: A new approach with future therapeutic potential.

Highlights: [•] To express functional photolyase in NHEK we applied a novel gene therapy method. [•] Our method is based on transfection of in vitro-transcribed, Ψ-modified mRNA. [•] The encoded protein repaired rapidly and efficiently the UVB-induced DNA lesions. [•] A novel approach with future therapeutic and research potential. [ABSTRACT FROM AUTHOR]