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Molecular cloning and expression of the hepatitis delta virus genotype IIb genome
- Source :
-
Biochemical & Biophysical Research Communications . Mar2003, Vol. 303 Issue 1, p357. 7p. - Publication Year :
- 2003
-
Abstract
- Analysis of hepatitis delta virus (HDV) genome sequences has revealed multiple genotypes with different geographical distributions and associated disease patterns. To date, replication-competent cDNA clones of HDV genotypes I, II, and III have been reported. HDV genotypes I, II, and IIb have been found in Taiwan. Although full-length sequences of genotype IIb have been published, its replication competence in cultured cells has yet to be reported. In order to examine this, we obtained a full-length cDNA clone, Taiwan-IIb-1, from a Taiwanese HDV genotype IIb isolate. Comparison of the complete nucleic acid sequence of Taiwan-IIb-1 with previously published genotype IIb isolates indicated that Taiwan-IIb-1 shares 98% identity with another Taiwanese isolate and 92% identity with a Japanese isolate. Transfection of Taiwan-IIb-1 into COS7 cells resulted in accumulation of the HDV genome and appearance of delta antigens, showing that cloned HDV genotype IIb can replicate in cultured cells. [Copyright &y& Elsevier]
- Subjects :
- *HEPATITIS
*VIRAL replication
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 303
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 9291327
- Full Text :
- https://doi.org/10.1016/S0006-291X(03)00338-3