Development of sandwich ELISA for detection and quantification of invertebrate major allergen tropomyosin by a monoclonal antibody.

Highlights: [•] A highly sensitive monoclonal antibody (MAb) for tropomyosins was produced. [•] The MAb specifically recognizes allergic tropomyosin-derived peptides. [•] A sandwich ELISA based on the MAb was developed. [•] The developed sandwich ELISA were successfully applied in processed foods. [ABSTRACT FROM AUTHOR]