Determination of three triterpene alcohols in rat plasma after oral administration of pollen of Brassica campestris based on the utilization of fetal bovine serum as surrogate matrix.

Highlights: [•] Fetal bovine serum was selected as the surrogate matrix and validated. [•] The derivatization of internal standard was developed and applied. [•] The method was developed for the first time. [•] The pharmacokinetics of DEH, MET and NOR was investigated. [•] This study provides the basis for the consumption of pollen of Brassica campestris. [Copyright &y& Elsevier]