Direct analysis of the mushroom poisons α- and β-amanitin in human urine using a novel on-line turbulent flow chromatography mode coupled to liquid chromatography–high resolution-mass spectrometry/mass spectrometry.

Highlights: [•] Introduction of a new TurboFlow (PQEM) mode for bioanalysis. [•] Simple, fast, and robust method for detection of α- and β-amanitin in human urine. [•] Application to analysis of authentic urine samples containing amatoxins. [•] Description of pseudo HR-MS3 studies as powerful tool in compound identification. [ABSTRACT FROM AUTHOR]