Role of residue lys315 in the mechanism of action of the Enterobacter cloacae 908R beta-lactamase.

Examines the role of the highly conserved lysine315 residue in the catalytic mechanism of a class C beta-lactamase. Use of site-directed mutagenesis; Effects of the mutations on the kinetics of penicillin G and cephalothin turnover; Pre-steady-state kinetics with carbenicillin at different pH; Activity at acidic pH.