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Genome-wide expressional and functional analysis of calcium transport elements during abiotic stress and development in rice.

Authors :
Singh, Amarjeet
Kanwar, Poonam
Yadav, Akhilesh K.
Mishra, Manali
Jha, Saroj K.
Baranwal, Vinay
Pandey, Amita
Kapoor, Sanjay
Tyagi, Akhilesh K.
Pandey, Girdhar K.
Source :
FEBS Journal. Feb2014, Vol. 281 Issue 3, p894-915. 22p.
Publication Year :
2014

Abstract

Ca2+ homeostasis is required to maintain a delicate balance of cytosolic Ca2+ during normal and adverse growth conditions. Various Ca2+ transporters actively participate to maintain this delicate balance especially during abiotic stresses and developmental events in plants. In this study, we present a genome-wide account, detailing expression profiles, subcellular localization and functional analysis of rice Ca2+ transport elements. Exhaustive in silico data mining and analysis resulted in the identification of 81 Ca2+ transport element genes, which belong to various groups such as Ca2+- ATPases (pumps), exchangers, channels, glutamate receptor homologs and annexins. Phylogenetic analysis revealed that different Ca2+ transporters are evolutionarily conserved across different plant species. Comprehensive expression analysis by gene chip microarray and quantitative RT- PCR revealed that a substantial proportion of Ca2+ transporter genes were expressed differentially under abiotic stresses (salt, cold and drought) and reproductive developmental stages (panicle and seed) in rice. These findings suggest a possible role of rice Ca2+ transporters in abiotic stress and development triggered signaling pathways. Subcellular localization of Ca2+ transporters from different groups in Nicotiana benthamiana revealed their variable localization to different compartments, which could be their possible sites of action. Complementation of Ca2+ transport activity of K616 yeast mutant by Ca2+- ATPase Os ACA7 and involvement in salt tolerance verified its functional behavior. This study will encourage detailed characterization of potential candidate Ca2+ transporters for their functional role in planta. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
1742464X
Volume :
281
Issue :
3
Database :
Academic Search Index
Journal :
FEBS Journal
Publication Type :
Academic Journal
Accession number :
94063214
Full Text :
https://doi.org/10.1111/febs.12656