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Biocatalytic production of 3′-sialyllactose by use of a modified sialidase with superior trans-sialidase activity.
- Source :
-
Process Biochemistry . Feb2014, Vol. 49 Issue 2, p265-270. 6p. - Publication Year :
- 2014
-
Abstract
- Highlights: [•] A mutated sialidase from Trypanosoma rangeli was expressed in Pichia pastoris. [•] The sialidase mutant was tuned for trans-sialidase activity by optimizing reaction conditions. [•] 3′-Sialyllactose and other sialylated HMO molecules were produced via trans-sialidase biocatalysis. [•] Casein glycomacropeptide was used as substrate for the trans-sialidase mutant biocatalysis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13595113
- Volume :
- 49
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Process Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 94304309
- Full Text :
- https://doi.org/10.1016/j.procbio.2013.10.023