Inhibition of Notch signaling leads to increased intracellular ROS by up-regulating Nox4 expression in primary HUVECs.

Highlights: [•] Blockade of Notch signaling increased reactive oxygen species (ROS) in primary HUVECs. [•] Abruption of ROS generation abolished Notch blockade-induced HUVEC proliferation, migration and adhesion. [•] The enhanced generation of ROS after blocking Notch signal was accompanied by augmented expression of Nox4. [•] Increased expression of Nox4 led to enhanced phosphorylation of VEGFR2 and ERK in HUVECs. [Copyright &y& Elsevier]