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Ultrasensitive nonenzymatic immunosensor based on bimetallic gold–silver nanoclusters synthesized by simple mortar grinding route.
- Source :
-
Sensors & Actuators B: Chemical . Apr2014, Vol. 194, p64-70. 7p. - Publication Year :
- 2014
-
Abstract
- Abstract: A simple nonenzymatic immunosensor was developed for sensitive and selective determination of squamous cell carcinoma antigen (SCC-Ag) based on bimetallic gold–silver nanoclusters (AuAg NCs) and reduced graphene oxide-tetraethylene pentamine (rGO-TEPA). rGO-TEPA was used as substrate material to immobilize antibodies since rGO-TEPA has large surface area and excellent conductivity to enhance the electrochemical response signal. AuAg NCs synthesized through simple mortar grinding route were used as catalytic labels of secondary antibodies; meanwhile, AuAg NCs could efficiently enhance the electron transfer. Under the optimal conditions, the electrochemical immunoassay exhibited a wide linear range of 0.005–20ng/mL with a low detection limit of 1.3pg/mL for SCC-Ag. The proposed sensing strategy enriches the electrochemical immunoassays and has a promising application in bioassay analysis. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 194
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 94407888
- Full Text :
- https://doi.org/10.1016/j.snb.2013.12.065