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Inter-laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191).
- Source :
-
Environmental Toxicology & Chemistry . Mar2014, Vol. 33 Issue 3, p662-670. 9p. - Publication Year :
- 2014
-
Abstract
- A whole-sediment toxicity test with Myriophyllum aquaticum has been developed by the German Federal Institute of Hydrology and standardized within the International Organization for Standardization (ISO; ISO 16191). An international ring-test was performed to evaluate the precision of the test method. Four sediments (artificial, natural) were tested. Test duration was 10 d, and test endpoint was inhibition of growth rate ( r) based on fresh weight data. Eighteen of 21 laboratories met the validity criterion of r ≥ 0.09 d−1 in the control. Results from 4 tests that did not conform to test-performance criteria were excluded from statistical evaluation. The inter-laboratory variability of growth rates (20.6%-25.0%) and inhibition (26.6%-39.9%) was comparable with the variability of other standardized bioassays. The mean test-internal variability of the controls was low (7% [control], 9.7% [solvent control]), yielding a high discriminatory power of the given test design (median minimum detectable differences [MDD] 13% to 15%). To ensure these MDDs, an additional validity criterion of CV ≤ 15% of the growth rate in the controls was recommended. As a positive control, 90 mg 3,5-dichlorophenol/kg sediment dry mass was tested. The range of the expected growth inhibition was proposed to be 35 ± 15%. The ring test results demonstrated the reliability of the ISO 16191 toxicity test and its suitability as a tool to assess the toxicity of sediment and dredged material. Environ Toxicol Chem 2014;33:662-670. © 2013 SETAC [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07307268
- Volume :
- 33
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Environmental Toxicology & Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 94447496
- Full Text :
- https://doi.org/10.1002/etc.2483